PixelSpacing = 1.778 Voltage = 300 ImageFile = TS_37.mrc ImageSize = 3708 3838 DataMode = 1 [T = SerialEM: Acquired by K2 on Caltech Krios 14-Sep-18 03:38:48 ] [T = Tilt axis angle = 86.2, binning = 1 spot = 7 camera = 0] [ZValue = 0] TiltAngle = 0.00739648 StagePosition = 577.078 -396.914 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.44736 ImageShift = -0.0452399 0.0412386 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -171 7362 105.752 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_0.0_Sep14_03.39.12.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:39:22 NavigatorLabel = 225 [ZValue = 1] TiltAngle = 2.98553 StagePosition = 577.078 -396.9 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.13878 ImageShift = -0.0137901 -0.0199563 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -189 7790 105.53 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_3.0_Sep14_03.40.40.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:40:50 NavigatorLabel = 225 [ZValue = 2] TiltAngle = -3.01023 StagePosition = 577.078 -396.919 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.22405 ImageShift = -0.0719177 0.117916 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -154 7506 105.385 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-3.0_Sep14_03.41.52.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:42:02 NavigatorLabel = 225 [ZValue = 3] TiltAngle = -6.01287 StagePosition = 577.078 -396.876 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.27482 ImageShift = -0.120966 0.22578 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -141 7296 105.188 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-6.0_Sep14_03.43.22.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:43:32 NavigatorLabel = 225 [ZValue = 4] TiltAngle = 5.98516 StagePosition = 577.078 -396.897 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.1079 ImageShift = 0.00237143 -0.0336181 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -89 7351 105.653 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_6.0_Sep14_03.44.24.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:44:34 NavigatorLabel = 225 [ZValue = 5] TiltAngle = 8.9843 StagePosition = 577.078 -396.897 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.10121 ImageShift = 0.00731682 -0.0491775 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -96 7266 105.545 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_9.0_Sep14_03.45.17.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:45:27 NavigatorLabel = 225 [ZValue = 6] TiltAngle = -9.013 StagePosition = 577.078 -396.89 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.35567 ImageShift = -0.0859628 0.171521 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -109 7242 104.871 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-9.0_Sep14_03.46.21.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:46:31 NavigatorLabel = 225 [ZValue = 7] TiltAngle = -12.0126 StagePosition = 577.078 -396.849 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.44058 ImageShift = -0.0912703 0.196591 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -112 7402 104.45 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-12.0_Sep14_03.47.24.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:47:35 NavigatorLabel = 225 [ZValue = 8] TiltAngle = 11.9839 StagePosition = 577.078 -396.895 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.04813 ImageShift = -0.0218719 0.0386021 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -121 7336 105.459 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_12.0_Sep14_03.48.45.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:48:55 NavigatorLabel = 225 [ZValue = 9] TiltAngle = 14.9836 StagePosition = 577.078 -396.895 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.00615 ImageShift = -0.0318763 0.0508439 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -193 7375 105.205 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_15.0_Sep14_03.49.38.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:49:48 NavigatorLabel = 225 [ZValue = 10] TiltAngle = -15.0118 StagePosition = 577.078 -396.892 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.43991 ImageShift = -0.0326578 0.104283 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -124 7409 103.953 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-15.0_Sep14_03.51.09.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:51:19 NavigatorLabel = 225 [ZValue = 11] TiltAngle = -18.0114 StagePosition = 577.078 -396.902 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.50403 ImageShift = -0.00989443 0.0784159 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -137 7208 103.264 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-18.0_Sep14_03.52.13.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:52:23 NavigatorLabel = 225 [ZValue = 12] TiltAngle = 17.9837 StagePosition = 577.078 -396.89 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.88432 ImageShift = -0.0570092 0.151188 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -175 7480 105.055 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_18.0_Sep14_03.53.33.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:53:43 NavigatorLabel = 225 [ZValue = 13] TiltAngle = 20.9858 StagePosition = 577.078 -396.897 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.84894 ImageShift = -0.0339158 0.0839627 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -153 7307 104.684 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_21.0_Sep14_03.54.34.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:54:44 NavigatorLabel = 225 [ZValue = 14] TiltAngle = -21.0105 StagePosition = 577.078 -396.905 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.43455 ImageShift = 0.0707397 -0.0603545 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -168 7167 102.635 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-21.0_Sep14_03.56.05.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:56:15 NavigatorLabel = 225 [ZValue = 15] TiltAngle = -24.0107 StagePosition = 577.078 -396.894 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.48534 ImageShift = 0.0775276 -0.0554481 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -97 7416 101.898 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-24.0_Sep14_03.57.00.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:57:10 NavigatorLabel = 225 [ZValue = 16] TiltAngle = 23.9835 StagePosition = 577.078 -396.882 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.66911 ImageShift = -0.0332238 0.124999 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -121 7305 104.066 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_24.0_Sep14_03.57.55.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:58:05 NavigatorLabel = 225 [ZValue = 17] TiltAngle = 26.9841 StagePosition = 577.078 -396.879 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.78786 ImageShift = 0.00339077 0.0271745 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -148 7401 103.755 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_27.0_Sep14_03.59.14.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 03:59:24 NavigatorLabel = 225 [ZValue = 18] TiltAngle = -27.0078 StagePosition = 577.078 -396.899 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.53436 ImageShift = 0.117537 -0.123692 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -188 7544 100.994 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-27.0_Sep14_04.00.19.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:00:29 NavigatorLabel = 225 [ZValue = 19] TiltAngle = -30.0099 StagePosition = 577.078 -396.851 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.63726 ImageShift = 0.112115 -0.0852943 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -127 7358 99.905 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-30.0_Sep14_04.01.22.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:01:32 NavigatorLabel = 225 [ZValue = 20] TiltAngle = 29.9832 StagePosition = 577.078 -396.881 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.63389 ImageShift = 0.00599383 0.0696993 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -180 7337 103.036 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_30.0_Sep14_04.02.17.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:02:27 NavigatorLabel = 225 [ZValue = 21] TiltAngle = 32.9834 StagePosition = 577.078 -396.882 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.63855 ImageShift = 0.0126606 0.0324412 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -123 7766 102.307 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_33.0_Sep14_04.03.10.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:03:20 NavigatorLabel = 225 [ZValue = 22] TiltAngle = -33.0071 StagePosition = 577.108 -396.873 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.63646 ImageShift = 0.140239 -0.122249 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -165 7432 98.7554 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-33.0_Sep14_04.04.16.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:04:26 NavigatorLabel = 225 [ZValue = 23] TiltAngle = -36.0092 StagePosition = 577.108 -396.873 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.75579 ImageShift = 0.127155 -0.0916436 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -104 7433 97.2767 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-36.0_Sep14_04.05.20.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:05:30 NavigatorLabel = 225 [ZValue = 24] TiltAngle = 35.983 StagePosition = 577.108 -396.867 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.52836 ImageShift = 0.0138146 0.0705157 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -148 7346 101.497 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_36.0_Sep14_04.06.15.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:06:26 NavigatorLabel = 225 [ZValue = 25] TiltAngle = 38.9826 StagePosition = 577.108 -396.867 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.52273 ImageShift = -0.0123453 0.0793634 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -93 7336 100.416 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_39.0_Sep14_04.07.17.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:07:27 NavigatorLabel = 225 [ZValue = 26] TiltAngle = -39.0083 StagePosition = 577.098 -396.866 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.57965 ImageShift = 0.175849 -0.193153 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -196 7323 95.7177 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-39.0_Sep14_04.08.50.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:09:00 NavigatorLabel = 225 [ZValue = 27] TiltAngle = -42.0085 StagePosition = 577.098 -396.866 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.69533 ImageShift = 0.173703 -0.170794 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -134 7421 93.9541 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-42.0_Sep14_04.09.36.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:09:46 NavigatorLabel = 225 [ZValue = 28] TiltAngle = 41.9838 StagePosition = 577.098 -396.843 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.29432 ImageShift = -0.0296767 0.14676 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -196 7639 99.2765 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_42.0_Sep14_04.10.40.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:10:51 NavigatorLabel = 225 [ZValue = 29] TiltAngle = 44.9849 StagePosition = 577.098 -396.841 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.26747 ImageShift = -0.0381673 0.135752 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -196 7431 97.982 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_45.0_Sep14_04.11.34.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:11:44 NavigatorLabel = 225 [ZValue = 30] TiltAngle = -45.0076 StagePosition = 577.049 -396.885 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.59415 ImageShift = 0.243906 -0.306984 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -104 7380 91.9631 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-45.0_Sep14_04.13.15.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:13:25 NavigatorLabel = 225 [ZValue = 31] TiltAngle = -48.0067 StagePosition = 577.049 -396.844 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.68276 ImageShift = 0.270136 -0.332497 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -186 7397 89.764 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-48.0_Sep14_04.14.10.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:14:20 NavigatorLabel = 225 [ZValue = 32] TiltAngle = 47.9855 StagePosition = 577.083 -396.85 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.09856 ImageShift = -0.0611785 0.161081 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -90 7275 96.5018 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_48.0_Sep14_04.15.15.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:15:25 NavigatorLabel = 225 [ZValue = 33] TiltAngle = 50.9837 StagePosition = 577.083 -396.85 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.06831 ImageShift = -0.0421894 0.0665624 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -163 7759 94.7254 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_51.0_Sep14_04.16.08.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:16:18 NavigatorLabel = 225 [ZValue = 34] TiltAngle = -51.0054 StagePosition = 577.063 -396.829 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.54067 ImageShift = 0.314905 -0.432092 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -146 7499 87.1252 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-51.0_Sep14_04.17.50.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:18:00 NavigatorLabel = 225 [ZValue = 35] TiltAngle = -54.0075 StagePosition = 577.063 -396.812 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.64868 ImageShift = 0.308794 -0.407571 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -208 7403 84.1927 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-54.0_Sep14_04.18.36.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:18:46 NavigatorLabel = 225 [ZValue = 36] TiltAngle = 53.9828 StagePosition = 577.084 -396.855 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 0.898775 ImageShift = -0.0366212 0.0282792 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -211 7734 92.9278 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_54.0_Sep14_04.19.50.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:20:00 NavigatorLabel = 225 [ZValue = 37] TiltAngle = 56.9814 StagePosition = 577.084 -396.855 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.16698 ImageShift = -0.0124339 -0.0963864 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -126 7442 90.909 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_57.0_Sep14_04.21.18.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:21:28 NavigatorLabel = 225 [ZValue = 38] TiltAngle = -57.0061 StagePosition = 577.1 -396.814 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.77111 ImageShift = 0.296583 -0.453316 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -168 7413 80.8824 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-57.0_Sep14_04.22.17.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:22:27 NavigatorLabel = 225 [ZValue = 39] TiltAngle = -60.0037 StagePosition = 577.08 -396.814 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.87608 ImageShift = 0.297877 -0.448508 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -190 7428 76.8838 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_-60.0_Sep14_04.23.20.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:23:30 NavigatorLabel = 225 [ZValue = 40] TiltAngle = 59.9845 StagePosition = 577.087 -396.838 StageZ = 83.6551 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 0.902262 ImageShift = -0.0132677 -0.148894 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -153 7468 88.2875 TargetDefocus = -2.75 SubFramePath = X:\KriosData_2\LaurenAnn\TS_37_60.0_Sep14_04.25.18.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 04:25:28 NavigatorLabel = 225