PixelSpacing = 1.778 Voltage = 300 ImageFile = TS_43.mrc ImageSize = 3708 3838 DataMode = 1 [T = SerialEM: Acquired by K2 on Caltech Krios 14-Sep-18 14:26:11 ] [T = Tilt axis angle = 86.2, binning = 1 spot = 7 camera = 0] [ZValue = 0] TiltAngle = 0.00739648 StagePosition = -502.158 -323.521 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.03852 ImageShift = -0.0450795 0.149654 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -106 7359 83.5343 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_0.0_Sep14_14.26.44.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:26:54 NavigatorLabel = 280 [ZValue = 1] TiltAngle = 2.98603 StagePosition = -502.158 -323.504 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.00119 ImageShift = -0.00193825 0.0475083 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -145 7364 83.5118 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_3.0_Sep14_14.28.20.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:28:31 NavigatorLabel = 280 [ZValue = 2] TiltAngle = -3.01273 StagePosition = -502.158 -323.516 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.09098 ImageShift = -0.0739048 0.189568 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -146 7554 83.6404 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-3.0_Sep14_14.29.52.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:30:02 NavigatorLabel = 280 [ZValue = 3] TiltAngle = -6.01287 StagePosition = -502.158 -323.475 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.18195 ImageShift = -0.109594 0.282226 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -177 7485 83.4542 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-6.0_Sep14_14.31.22.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:31:32 NavigatorLabel = 280 [ZValue = 4] TiltAngle = 5.98616 StagePosition = -502.158 -323.509 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.00459 ImageShift = 0.000888604 0.0343912 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -120 7490 83.4444 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_6.0_Sep14_14.32.16.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:32:27 NavigatorLabel = 280 [ZValue = 5] TiltAngle = 8.9853 StagePosition = -502.158 -323.509 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.01256 ImageShift = -0.00693859 0.0443812 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -160 7387 83.0731 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_9.0_Sep14_14.33.28.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:33:38 NavigatorLabel = 280 [ZValue = 6] TiltAngle = -9.013 StagePosition = -502.158 -323.504 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.24111 ImageShift = -0.0805693 0.233305 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -186 7618 83.2658 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-9.0_Sep14_14.34.42.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:34:53 NavigatorLabel = 280 [ZValue = 7] TiltAngle = -12.0121 StagePosition = -502.158 -323.467 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.32546 ImageShift = -0.0794372 0.245205 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -122 7385 82.8248 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-12.0_Sep14_14.35.38.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:35:48 NavigatorLabel = 280 [ZValue = 8] TiltAngle = 11.9839 StagePosition = -502.158 -323.506 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.931 ImageShift = -0.0371601 0.137005 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -150 7559 82.6133 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_12.0_Sep14_14.36.58.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:37:09 NavigatorLabel = 280 [ZValue = 9] TiltAngle = 14.9841 StagePosition = -502.158 -323.498 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.92701 ImageShift = -0.0440852 0.134949 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -118 7450 82.0096 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_15.0_Sep14_14.38.01.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:38:11 NavigatorLabel = 280 [ZValue = 10] TiltAngle = -15.0118 StagePosition = -502.158 -323.529 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.30947 ImageShift = -0.0266412 0.160179 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -94 7439 82.2565 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-15.0_Sep14_14.39.23.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:39:33 NavigatorLabel = 280 [ZValue = 11] TiltAngle = -18.0114 StagePosition = -502.158 -323.455 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.3609 ImageShift = -0.00124793 0.130963 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -174 7318 81.6785 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-18.0_Sep14_14.40.36.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:40:47 NavigatorLabel = 280 [ZValue = 12] TiltAngle = 17.9837 StagePosition = -502.158 -323.477 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.77311 ImageShift = -0.0731542 0.239793 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -203 7362 81.3412 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_18.0_Sep14_14.41.57.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:42:07 NavigatorLabel = 280 [ZValue = 13] TiltAngle = 20.9838 StagePosition = -502.158 -323.474 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.74901 ImageShift = -0.0745021 0.22553 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -137 7422 80.4383 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_21.0_Sep14_14.42.51.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:43:01 NavigatorLabel = 280 [ZValue = 14] TiltAngle = -21.011 StagePosition = -502.158 -323.43 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.3274 ImageShift = 0.0531266 0.0505644 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -194 7297 80.9773 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-21.0_Sep14_14.44.14.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:44:24 NavigatorLabel = 280 [ZValue = 15] TiltAngle = -24.0107 StagePosition = -502.158 -323.44 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.38333 ImageShift = 0.0491574 0.071411 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -152 7234 79.9544 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-24.0_Sep14_14.45.01.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:45:11 NavigatorLabel = 280 [ZValue = 16] TiltAngle = 23.985 StagePosition = -502.158 -323.424 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.57877 ImageShift = -0.0642329 0.245198 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -184 7241 79.4109 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_24.0_Sep14_14.45.57.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:46:07 NavigatorLabel = 280 [ZValue = 17] TiltAngle = 26.9846 StagePosition = -502.158 -323.425 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.57338 ImageShift = -0.0515076 0.196116 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -149 7367 78.1754 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_27.0_Sep14_14.46.51.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:47:02 NavigatorLabel = 280 [ZValue = 18] TiltAngle = -27.0103 StagePosition = -502.193 -323.409 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.3857 ImageShift = 0.091129 0.00817693 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -175 7408 78.8432 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-27.0_Sep14_14.47.58.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:48:08 NavigatorLabel = 280 [ZValue = 19] TiltAngle = -30.0099 StagePosition = -502.193 -323.374 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.47365 ImageShift = 0.0945466 0.0385453 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -156 7315 77.5444 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-30.0_Sep14_14.49.20.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:49:30 NavigatorLabel = 280 [ZValue = 20] TiltAngle = 29.9867 StagePosition = -502.193 -323.432 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.48885 ImageShift = -0.0273333 0.19405 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -178 7529 76.8812 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_30.0_Sep14_14.50.08.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:50:18 NavigatorLabel = 280 [ZValue = 21] TiltAngle = 32.9834 StagePosition = -502.193 -323.432 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.49667 ImageShift = -0.026833 0.187049 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -156 7412 75.3893 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_33.0_Sep14_14.51.10.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:51:20 NavigatorLabel = 280 [ZValue = 22] TiltAngle = -33.0096 StagePosition = -502.259 -323.422 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.47466 ImageShift = 0.136145 0.021155 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -152 7475 76.2375 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-33.0_Sep14_14.52.17.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:52:27 NavigatorLabel = 280 [ZValue = 23] TiltAngle = -36.0097 StagePosition = -502.259 -323.419 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.55824 ImageShift = 0.169133 0.0330003 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -112 7457 74.427 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-36.0_Sep14_14.53.30.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:53:40 NavigatorLabel = 280 [ZValue = 24] TiltAngle = 35.9825 StagePosition = -502.259 -323.435 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.38887 ImageShift = -0.00149749 0.262052 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -179 7509 73.6564 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_36.0_Sep14_14.54.27.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:54:37 NavigatorLabel = 280 [ZValue = 25] TiltAngle = 38.9846 StagePosition = -502.259 -323.435 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.37765 ImageShift = -0.0064638 0.28024 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -105 7414 71.8549 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_39.0_Sep14_14.55.47.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:55:58 NavigatorLabel = 280 [ZValue = 26] TiltAngle = -39.0093 StagePosition = -502.307 -323.424 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.4772 ImageShift = 0.253188 -0.0579987 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -130 7422 72.8075 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-39.0_Sep14_14.57.20.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:57:30 NavigatorLabel = 280 [ZValue = 27] TiltAngle = -42.009 StagePosition = -502.307 -323.424 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.58542 ImageShift = 0.264338 -0.0422961 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -148 7251 70.9664 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-42.0_Sep14_14.58.16.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:58:26 NavigatorLabel = 280 [ZValue = 28] TiltAngle = 41.9828 StagePosition = -502.307 -323.419 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.24007 ImageShift = 0.0190404 0.335031 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -201 7450 69.5912 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_42.0_Sep14_14.59.14.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 14:59:24 NavigatorLabel = 280 [ZValue = 29] TiltAngle = 44.9844 StagePosition = -502.307 -323.441 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.20497 ImageShift = 0.0196364 0.334773 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -114 7388 67.072 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_45.0_Sep14_15.00.26.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:00:36 NavigatorLabel = 280 [ZValue = 30] TiltAngle = -45.0071 StagePosition = -502.376 -323.444 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.50774 ImageShift = 0.364061 -0.156736 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -146 7481 68.5833 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-45.0_Sep14_15.01.58.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:02:08 NavigatorLabel = 280 [ZValue = 31] TiltAngle = -48.0077 StagePosition = -502.376 -323.434 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.55375 ImageShift = 0.396614 -0.170311 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -151 7281 65.9032 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-48.0_Sep14_15.03.03.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:03:13 NavigatorLabel = 280 [ZValue = 32] TiltAngle = 47.982 StagePosition = -502.376 -323.439 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.04487 ImageShift = 0.0700759 0.380169 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -166 7290 64.2038 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_48.0_Sep14_15.04.10.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:04:20 NavigatorLabel = 280 [ZValue = 33] TiltAngle = 50.9851 StagePosition = -502.376 -323.436 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 2.21229 ImageShift = 0.112227 0.296823 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -162 7463 61.248 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_51.0_Sep14_15.05.45.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:05:55 NavigatorLabel = 280 [ZValue = 34] TiltAngle = -51.0079 StagePosition = -502.466 -323.484 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.46804 ImageShift = 0.498748 -0.256214 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -181 7489 62.896 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-51.0_Sep14_15.07.31.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:07:41 NavigatorLabel = 280 [ZValue = 35] TiltAngle = -54.0075 StagePosition = -502.466 -323.397 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.46814 ImageShift = 0.535999 -0.256068 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -184 7434 59.7295 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-54.0_Sep14_15.08.53.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:09:03 NavigatorLabel = 280 [ZValue = 36] TiltAngle = 53.9843 StagePosition = -502.403 -323.361 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.95642 ImageShift = 0.152824 0.270229 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -185 7430 57.5171 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_54.0_Sep14_15.10.00.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:10:10 NavigatorLabel = 280 [ZValue = 37] TiltAngle = 56.9804 StagePosition = -502.403 -323.361 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.83948 ImageShift = 0.152798 0.2413 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -142 7336 53.6662 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_57.0_Sep14_15.10.54.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:11:05 NavigatorLabel = 280 [ZValue = 38] TiltAngle = -57.0066 StagePosition = -502.411 -323.446 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.52751 ImageShift = 0.543395 -0.241333 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -125 7429 56.1365 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-57.0_Sep14_15.11.46.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:11:56 NavigatorLabel = 280 [ZValue = 39] TiltAngle = -60.0048 StagePosition = -502.411 -323.449 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 3.58347 ImageShift = 0.555551 -0.282482 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -165 7332 51.9499 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_-60.0_Sep14_15.12.51.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:13:01 NavigatorLabel = 280 [ZValue = 40] TiltAngle = 59.9825 StagePosition = -502.389 -323.409 StageZ = 103.225 Magnification = 81000 Intensity = 0.109056 ExposureDose = 0 PixelSpacing = 1.778 SpotSize = 7 Defocus = 1.79794 ImageShift = 0.166022 0.163273 RotationAngle = 176.2 ExposureTime = 1.3 Binning = 1 CameraIndex = 0 DividedBy2 = 1 MagIndex = 30 CountsPerElectron = 17.91 MinMaxMean = -213 7429 49.1855 TargetDefocus = -4.25 SubFramePath = X:\KriosData_2\LaurenAnn\TS_43_60.0_Sep14_15.14.07.mrcs NumSubFrames = 13 FrameDosesAndNumber = 0 13 DateTime = 14-Sep-18 15:14:18 NavigatorLabel = 280