PixelSpacing = 3.36 Voltage = 300 ImageFile = ts3.mrc ImageSize = 3936 2794 DataMode = 1 [T = SerialEM: Acquired by K3 on Caltech Krios 23-Jun-19 11:44:45 ] [T = Tilt axis angle = 85.5, binning = 1 spot = 6 camera = 0 bidir = 0.0] [ZValue = 0] TiltAngle = -37.9865 StagePosition = 447.588 439.514 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -7.20751 ImageShift = 0.223261 -0.103577 RotationAngle = 175.47 ExposureTime = 0.756328 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -30 1018 25.1926 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:44:27 FilterSlitAndLoss = 20 0 [ZValue = 1] TiltAngle = -35.9872 StagePosition = 447.598 439.502 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.6825 ImageShift = 0.222894 -0.103891 RotationAngle = 175.47 ExposureTime = 0.735022 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -29 338 29.3603 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:44:00 FilterSlitAndLoss = 20 0 [ZValue = 2] TiltAngle = -33.9869 StagePosition = 447.609 439.488 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.6996 ImageShift = 0.209623 -0.0845065 RotationAngle = 175.47 ExposureTime = 0.72437 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 478 31.7186 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:43:55 FilterSlitAndLoss = 20 0 [ZValue = 3] TiltAngle = -31.9882 StagePosition = 447.61 439.485 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7167 ImageShift = 0.197123 -0.074384 RotationAngle = 175.47 ExposureTime = 0.703065 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 280 32.5941 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:43:40 FilterSlitAndLoss = 20 0 [ZValue = 4] TiltAngle = -29.9894 StagePosition = 447.607 439.48 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7306 ImageShift = 0.201221 -0.0721399 RotationAngle = 175.47 ExposureTime = 0.692412 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -31 275 33.6617 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:43:36 FilterSlitAndLoss = 20 0 [ZValue = 5] TiltAngle = -27.9882 StagePosition = 447.611 439.476 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.8161 ImageShift = 0.206677 -0.0927221 RotationAngle = 175.47 ExposureTime = 0.671108 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -32 220 34.1007 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:43:16 FilterSlitAndLoss = 20 0 [ZValue = 6] TiltAngle = -25.9889 StagePosition = 447.614 439.473 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.8091 ImageShift = 0.202397 -0.0943397 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -38 196 34.8207 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:43:12 FilterSlitAndLoss = 20 0 [ZValue = 7] TiltAngle = -23.9907 StagePosition = 447.621 439.478 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7935 ImageShift = 0.207787 -0.101306 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -30 231 35.5484 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:43:07 FilterSlitAndLoss = 20 0 [ZValue = 8] TiltAngle = -21.9879 StagePosition = 447.619 439.455 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.8271 ImageShift = 0.212718 -0.0981096 RotationAngle = 175.47 ExposureTime = 0.63915 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 266 36.0695 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:42:47 FilterSlitAndLoss = 20 0 [ZValue = 9] TiltAngle = -19.9882 StagePosition = 447.621 439.452 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.8145 ImageShift = 0.198932 -0.0889873 RotationAngle = 175.47 ExposureTime = 0.63915 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 304 36.9849 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:42:33 FilterSlitAndLoss = 20 0 [ZValue = 10] TiltAngle = -17.9904 StagePosition = 447.622 439.444 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7639 ImageShift = 0.204961 -0.115942 RotationAngle = 175.47 ExposureTime = 0.628498 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -31 401 37.3073 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:42:06 FilterSlitAndLoss = 20 0 [ZValue = 11] TiltAngle = -15.9871 StagePosition = 447.624 439.459 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7704 ImageShift = 0.204297 -0.175786 RotationAngle = 175.47 ExposureTime = 0.617845 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -31 214 37.5342 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:41:40 FilterSlitAndLoss = 20 0 [ZValue = 12] TiltAngle = -13.9909 StagePosition = 447.63 439.451 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7028 ImageShift = 0.228683 -0.221774 RotationAngle = 175.47 ExposureTime = 0.617845 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 285 38.6266 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:40:58 FilterSlitAndLoss = 20 0 [ZValue = 13] TiltAngle = -11.9911 StagePosition = 447.638 439.453 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7065 ImageShift = 0.208313 -0.193378 RotationAngle = 175.47 ExposureTime = 0.607193 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 204 38.5308 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:40:54 FilterSlitAndLoss = 20 0 [ZValue = 14] TiltAngle = -9.99038 StagePosition = 447.64 439.457 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7109 ImageShift = 0.191807 -0.164182 RotationAngle = 175.47 ExposureTime = 0.607193 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 314 39.2212 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:40:49 FilterSlitAndLoss = 20 0 [ZValue = 15] TiltAngle = -7.99062 StagePosition = 447.642 439.458 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7483 ImageShift = 0.194833 -0.180272 RotationAngle = 175.47 ExposureTime = 0.607193 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 268 39.7765 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:40:29 FilterSlitAndLoss = 20 0 [ZValue = 16] TiltAngle = -5.99137 StagePosition = 447.64 439.458 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7159 ImageShift = 0.173014 -0.145186 RotationAngle = 175.47 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 230 39.3247 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:40:12 FilterSlitAndLoss = 20 0 [ZValue = 17] TiltAngle = -3.99111 StagePosition = 447.639 439.456 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.7229 ImageShift = 0.169012 -0.150915 RotationAngle = 175.47 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 366 39.6388 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:39:46 FilterSlitAndLoss = 20 0 [ZValue = 18] TiltAngle = -1.99186 StagePosition = 447.64 439.461 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.6707 ImageShift = 0.159568 -0.132566 RotationAngle = 175.47 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -32 231 40.0124 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:39:20 FilterSlitAndLoss = 20 0 [ZValue = 19] TiltAngle = 0.00789641 StagePosition = 447.613 439.576 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.4631 ImageShift = 0.0971208 -0.0375834 RotationAngle = 175.47 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -31 410 40.5092 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:38:59 FilterSlitAndLoss = 20 0 [ZValue = 20] TiltAngle = 1.99165 StagePosition = 447.579 439.641 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.129 ImageShift = 0.312841 -0.333156 RotationAngle = 175.47 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 281 40.0985 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:46:05 FilterSlitAndLoss = 20 0 [ZValue = 21] TiltAngle = 3.99141 StagePosition = 447.579 439.632 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.0179 ImageShift = 0.322547 -0.350894 RotationAngle = 175.47 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -32 355 40.2006 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:46:31 FilterSlitAndLoss = 20 0 [ZValue = 22] TiltAngle = 5.99116 StagePosition = 447.577 439.625 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.0299 ImageShift = 0.330668 -0.360359 RotationAngle = 175.47 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -32 211 40.1742 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:46:57 FilterSlitAndLoss = 20 0 [ZValue = 23] TiltAngle = 7.99092 StagePosition = 447.578 439.623 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.9598 ImageShift = 0.315253 -0.313823 RotationAngle = 175.47 ExposureTime = 0.607193 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -32 210 40.4254 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:47:12 FilterSlitAndLoss = 20 0 [ZValue = 24] TiltAngle = 9.99117 StagePosition = 447.582 439.621 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.0533 ImageShift = 0.328087 -0.335305 RotationAngle = 175.47 ExposureTime = 0.607193 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 285 40.2988 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:47:31 FilterSlitAndLoss = 20 0 [ZValue = 25] TiltAngle = 11.9924 StagePosition = 447.58 439.624 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.0651 ImageShift = 0.333423 -0.352055 RotationAngle = 175.47 ExposureTime = 0.607193 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 225 40.1059 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:47:36 FilterSlitAndLoss = 20 0 [ZValue = 26] TiltAngle = 13.9932 StagePosition = 447.579 439.624 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.0769 ImageShift = 0.326468 -0.340849 RotationAngle = 175.47 ExposureTime = 0.617845 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 202 40.2686 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:47:41 FilterSlitAndLoss = 20 0 [ZValue = 27] TiltAngle = 15.9919 StagePosition = 447.584 439.63 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.133 ImageShift = 0.330486 -0.345613 RotationAngle = 175.47 ExposureTime = 0.617845 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 449 39.9297 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:48:00 FilterSlitAndLoss = 20 0 [ZValue = 28] TiltAngle = 17.9942 StagePosition = 447.579 439.634 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.1494 ImageShift = 0.324442 -0.35167 RotationAngle = 175.47 ExposureTime = 0.628498 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -32 212 39.9945 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:48:14 FilterSlitAndLoss = 20 0 [ZValue = 29] TiltAngle = 19.9939 StagePosition = 447.575 439.638 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.1705 ImageShift = 0.318843 -0.326105 RotationAngle = 175.47 ExposureTime = 0.63915 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -31 272 39.9868 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:48:19 FilterSlitAndLoss = 20 0 [ZValue = 30] TiltAngle = 21.9932 StagePosition = 447.577 439.644 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.1916 ImageShift = 0.308783 -0.302234 RotationAngle = 175.47 ExposureTime = 0.63915 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 247 39.1381 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:48:24 FilterSlitAndLoss = 20 0 [ZValue = 31] TiltAngle = 23.993 StagePosition = 447.568 439.645 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.0863 ImageShift = 0.292405 -0.279446 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 259 38.8254 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:48:43 FilterSlitAndLoss = 20 0 [ZValue = 32] TiltAngle = 25.9927 StagePosition = 447.563 439.648 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.1088 ImageShift = 0.289001 -0.280084 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 242 38.072 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:48:47 FilterSlitAndLoss = 20 0 [ZValue = 33] TiltAngle = 27.9935 StagePosition = 447.57 439.654 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.1126 ImageShift = 0.278382 -0.251055 RotationAngle = 175.47 ExposureTime = 0.671108 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 273 37.2447 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:49:02 FilterSlitAndLoss = 20 0 [ZValue = 34] TiltAngle = 29.9937 StagePosition = 447.566 439.655 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.1164 ImageShift = 0.270631 -0.242294 RotationAngle = 175.47 ExposureTime = 0.692412 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 427 36.7663 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:49:06 FilterSlitAndLoss = 20 0 [ZValue = 35] TiltAngle = 31.979 StagePosition = 447.57 439.646 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.199 ImageShift = 0.274088 -0.246772 RotationAngle = 175.47 ExposureTime = 0.703065 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -32 200 35.0435 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:49:48 FilterSlitAndLoss = 20 0 [ZValue = 36] TiltAngle = 33.9927 StagePosition = 447.564 439.662 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.2051 ImageShift = 0.307605 -0.27716 RotationAngle = 175.47 ExposureTime = 0.72437 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 203 31.8676 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:50:15 FilterSlitAndLoss = 20 0 [ZValue = 37] TiltAngle = 35.993 StagePosition = 447.536 439.666 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.3211 ImageShift = 0.311187 -0.281303 RotationAngle = 175.47 ExposureTime = 0.735022 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -30 398 27.6738 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:50:42 FilterSlitAndLoss = 20 0 [ZValue = 38] TiltAngle = 37.9817 StagePosition = 447.601 439.658 StageZ = 106.999 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -11.3853 ImageShift = 0.239561 -0.118341 RotationAngle = 175.47 ExposureTime = 0.756328 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -30 320 27.714 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 11:51:21 FilterSlitAndLoss = 20 0