PixelSpacing = 3.36 Voltage = 300 ImageFile = SquThreeTS.mrc ImageSize = 5760 4092 DataMode = 1 [T = SerialEM: Acquired by K3 on Caltech Krios 23-Jun-19 10:05:37 ] [T = Tilt axis angle = 85.5, binning = 1 spot = 6 camera = 0 bidir = 0.0] [ZValue = 0] TiltAngle = -59.9863 StagePosition = -215.566 -316.252 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -7.46767 ImageShift = -0.229933 0.456612 RotationAngle = 175.47 ExposureTime = 1.29961 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 597 62.2442 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:05:37 FilterSlitAndLoss = 20 0 [ZValue = 1] TiltAngle = -57.987 StagePosition = -215.565 -316.255 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -7.61754 ImageShift = -0.342118 0.667088 RotationAngle = 175.47 ExposureTime = 1.22504 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 647 68.4811 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:05:18 FilterSlitAndLoss = 20 0 [ZValue = 2] TiltAngle = -55.9867 StagePosition = -215.568 -316.254 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -7.77097 ImageShift = -0.28887 0.568586 RotationAngle = 175.47 ExposureTime = 1.16112 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 605 65.6984 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:05:05 FilterSlitAndLoss = 20 0 [ZValue = 3] TiltAngle = -53.9875 StagePosition = -215.568 -316.298 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -7.7856 ImageShift = -0.362221 0.69966 RotationAngle = 175.47 ExposureTime = 1.10786 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -41 552 70.4277 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:04:37 FilterSlitAndLoss = 20 0 [ZValue = 4] TiltAngle = -51.9867 StagePosition = -215.571 -316.304 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -7.98821 ImageShift = -0.326616 0.643995 RotationAngle = 175.47 ExposureTime = 1.0546 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 521 68.1022 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:04:24 FilterSlitAndLoss = 20 0 [ZValue = 5] TiltAngle = -49.986 StagePosition = -215.55 -316.314 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -8.20273 ImageShift = -0.278151 0.555571 RotationAngle = 175.47 ExposureTime = 1.01199 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 776 67.0433 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:04:12 FilterSlitAndLoss = 20 0 [ZValue = 6] TiltAngle = -47.9867 StagePosition = -215.534 -316.314 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -8.37758 ImageShift = -0.233717 0.472176 RotationAngle = 175.47 ExposureTime = 0.969378 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -40 513 65.7708 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:03:49 FilterSlitAndLoss = 20 0 [ZValue = 7] TiltAngle = -45.9875 StagePosition = -215.543 -316.316 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -8.92761 ImageShift = -0.180386 0.390581 RotationAngle = 175.47 ExposureTime = 0.93742 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 470 65.0762 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:03:20 FilterSlitAndLoss = 20 0 [ZValue = 8] TiltAngle = -43.9887 StagePosition = -215.531 -316.322 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -8.96436 ImageShift = -0.127959 0.316674 RotationAngle = 175.47 ExposureTime = 0.905463 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -33 466 64.2471 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:03:07 FilterSlitAndLoss = 20 0 [ZValue = 9] TiltAngle = -41.987 StagePosition = -215.516 -316.323 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.00527 ImageShift = -0.035258 0.164763 RotationAngle = 175.47 ExposureTime = 0.873505 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 436 62.1756 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:02:55 FilterSlitAndLoss = 20 0 [ZValue = 10] TiltAngle = -39.9877 StagePosition = -215.525 -316.326 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -8.99987 ImageShift = -0.0453989 0.186526 RotationAngle = 175.47 ExposureTime = 0.8522 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -42 622 63.8079 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:02:30 FilterSlitAndLoss = 20 0 [ZValue = 11] TiltAngle = -37.9869 StagePosition = -215.519 -316.326 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.04788 ImageShift = -0.0600275 0.204607 RotationAngle = 175.47 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -41 443 64.0887 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:02:26 FilterSlitAndLoss = 20 0 [ZValue = 12] TiltAngle = -35.9877 StagePosition = -215.5 -316.324 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.103 ImageShift = -0.0572822 0.209184 RotationAngle = 175.47 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -40 413 64.4373 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:02:21 FilterSlitAndLoss = 20 0 [ZValue = 13] TiltAngle = -33.9879 StagePosition = -215.497 -316.324 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.1629 ImageShift = -0.073745 0.234124 RotationAngle = 175.47 ExposureTime = 0.788285 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 437 66.0476 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:02:00 FilterSlitAndLoss = 20 0 [ZValue = 14] TiltAngle = -31.9902 StagePosition = -215.492 -316.316 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.20222 ImageShift = -0.0867598 0.249827 RotationAngle = 175.47 ExposureTime = 0.76698 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -44 570 66.465 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:01:44 FilterSlitAndLoss = 20 0 [ZValue = 15] TiltAngle = -29.9904 StagePosition = -215.476 -316.304 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.22574 ImageShift = -0.0996313 0.270496 RotationAngle = 175.47 ExposureTime = 0.745675 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -38 421 66.5112 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:01:40 FilterSlitAndLoss = 20 0 [ZValue = 16] TiltAngle = -27.9902 StagePosition = -215.462 -316.347 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.33857 ImageShift = -0.0949625 0.256789 RotationAngle = 175.47 ExposureTime = 0.735022 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -43 416 66.972 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:01:19 FilterSlitAndLoss = 20 0 [ZValue = 17] TiltAngle = -25.9894 StagePosition = -215.453 -316.343 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.36392 ImageShift = -0.0960936 0.257852 RotationAngle = 175.47 ExposureTime = 0.72437 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 430 67.7183 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:01:14 FilterSlitAndLoss = 20 0 [ZValue = 18] TiltAngle = -23.9897 StagePosition = -215.469 -316.359 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.39109 ImageShift = -0.0904873 0.251813 RotationAngle = 175.47 ExposureTime = 0.713718 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 448 67.8753 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:01:09 FilterSlitAndLoss = 20 0 [ZValue = 19] TiltAngle = -21.9899 StagePosition = -215.474 -316.344 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.40432 ImageShift = -0.100733 0.257114 RotationAngle = 175.47 ExposureTime = 0.703065 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -42 439 68.5273 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:00:44 FilterSlitAndLoss = 20 0 [ZValue = 20] TiltAngle = -19.9892 StagePosition = -215.462 -316.366 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.43249 ImageShift = -0.0987272 0.249817 RotationAngle = 175.47 ExposureTime = 0.692412 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 426 68.4965 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:00:40 FilterSlitAndLoss = 20 0 [ZValue = 21] TiltAngle = -17.9904 StagePosition = -215.465 -316.37 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.46165 ImageShift = -0.083 0.221527 RotationAngle = 175.47 ExposureTime = 0.68176 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 438 67.9778 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:00:35 FilterSlitAndLoss = 20 0 [ZValue = 22] TiltAngle = -15.9911 StagePosition = -215.455 -316.365 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.48405 ImageShift = -0.0889674 0.213299 RotationAngle = 175.47 ExposureTime = 0.671108 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 438 68.2659 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:00:14 FilterSlitAndLoss = 20 0 [ZValue = 23] TiltAngle = -13.9899 StagePosition = -215.456 -316.36 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.51352 ImageShift = -0.100333 0.234286 RotationAngle = 175.47 ExposureTime = 0.671108 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 431 69.1193 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:00:09 FilterSlitAndLoss = 20 0 [ZValue = 24] TiltAngle = -11.9891 StagePosition = -215.452 -316.357 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.54316 ImageShift = -0.10463 0.232577 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 571 68.6485 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 09:59:57 FilterSlitAndLoss = 20 0 [ZValue = 25] TiltAngle = -9.98888 StagePosition = -215.451 -316.351 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.56826 ImageShift = -0.118227 0.261613 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -38 605 69.4171 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 09:59:35 FilterSlitAndLoss = 20 0 [ZValue = 26] TiltAngle = -7.99062 StagePosition = -215.454 -316.337 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.59939 ImageShift = -0.117187 0.248877 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 493 69.4986 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 09:59:30 FilterSlitAndLoss = 20 0 [ZValue = 27] TiltAngle = -5.99087 StagePosition = -215.459 -316.316 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.63064 ImageShift = -0.116776 0.239492 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 425 68.7768 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 09:59:18 FilterSlitAndLoss = 20 0 [ZValue = 28] TiltAngle = -3.99061 StagePosition = -215.454 -316.337 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.66139 ImageShift = -0.115532 0.224114 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -41 482 68.8539 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 09:58:55 FilterSlitAndLoss = 20 0 [ZValue = 29] TiltAngle = -1.99186 StagePosition = -215.45 -316.261 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.69289 ImageShift = -0.122052 0.225212 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 407 69.033 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 09:58:33 FilterSlitAndLoss = 20 0 [ZValue = 30] TiltAngle = 0.00939623 StagePosition = -215.474 -316.23 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.57006 ImageShift = -0.0385296 0.0125848 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -38 428 69.338 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 09:58:10 FilterSlitAndLoss = 20 0 [ZValue = 31] TiltAngle = 1.99365 StagePosition = -215.513 -316.104 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.79872 ImageShift = -0.0367999 0.155584 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -43 434 69.673 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:07:06 FilterSlitAndLoss = 20 0 [ZValue = 32] TiltAngle = 3.99341 StagePosition = -215.512 -316.099 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.70519 ImageShift = -0.0393811 0.161926 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 403 69.6829 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:07:28 FilterSlitAndLoss = 20 0 [ZValue = 33] TiltAngle = 5.99116 StagePosition = -215.508 -316.103 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.73824 ImageShift = -0.037994 0.157144 RotationAngle = 175.47 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -41 411 69.3854 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:07:50 FilterSlitAndLoss = 20 0 [ZValue = 34] TiltAngle = 7.99142 StagePosition = -215.51 -316.099 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.6869 ImageShift = -0.0248265 0.131626 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 508 70.133 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:07:55 FilterSlitAndLoss = 20 0 [ZValue = 35] TiltAngle = 9.99367 StagePosition = -215.512 -316.096 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.76733 ImageShift = -0.0126551 0.109382 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -44 409 69.4025 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:08:16 FilterSlitAndLoss = 20 0 [ZValue = 36] TiltAngle = 11.9919 StagePosition = -215.508 -316.089 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.78981 ImageShift = -0.00643158 0.0899867 RotationAngle = 175.47 ExposureTime = 0.660455 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 432 68.7623 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:08:21 FilterSlitAndLoss = 20 0 [ZValue = 37] TiltAngle = 13.9952 StagePosition = -215.509 -316.084 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.80964 ImageShift = -0.0117103 0.100577 RotationAngle = 175.47 ExposureTime = 0.671108 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -38 417 69.2722 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:08:26 FilterSlitAndLoss = 20 0 [ZValue = 38] TiltAngle = 15.9934 StagePosition = -215.511 -316.073 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.82947 ImageShift = -0.0110362 0.0996445 RotationAngle = 175.47 ExposureTime = 0.671108 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 440 68.3479 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:08:30 FilterSlitAndLoss = 20 0 [ZValue = 39] TiltAngle = 17.9912 StagePosition = -215.51 -316.063 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.8488 ImageShift = -0.0122273 0.0909388 RotationAngle = 175.47 ExposureTime = 0.68176 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 548 68.5708 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:08:54 FilterSlitAndLoss = 20 0 [ZValue = 40] TiltAngle = 19.9909 StagePosition = -215.513 -316.055 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.86584 ImageShift = -0.0216987 0.113597 RotationAngle = 175.47 ExposureTime = 0.692412 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -35 422 68.7203 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:08:59 FilterSlitAndLoss = 20 0 [ZValue = 41] TiltAngle = 21.9902 StagePosition = -215.516 -316.042 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.88455 ImageShift = -0.0314774 0.138316 RotationAngle = 175.47 ExposureTime = 0.703065 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 436 68.4328 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:09:04 FilterSlitAndLoss = 20 0 [ZValue = 42] TiltAngle = 23.9895 StagePosition = -215.513 -316.031 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.90325 ImageShift = -0.0563413 0.162401 RotationAngle = 175.47 ExposureTime = 0.713718 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -42 434 68.6082 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:09:09 FilterSlitAndLoss = 20 0 [ZValue = 43] TiltAngle = 25.9907 StagePosition = -215.522 -316.024 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.88541 ImageShift = -0.0612825 0.170778 RotationAngle = 175.47 ExposureTime = 0.72437 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 419 67.9397 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:09:29 FilterSlitAndLoss = 20 0 [ZValue = 44] TiltAngle = 27.9875 StagePosition = -215.535 -316.016 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.90764 ImageShift = -0.0661616 0.184683 RotationAngle = 175.47 ExposureTime = 0.735022 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 447 67.5101 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:09:45 FilterSlitAndLoss = 20 0 [ZValue = 45] TiltAngle = 29.9922 StagePosition = -215.514 -316.006 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.9224 ImageShift = -0.0676652 0.191643 RotationAngle = 175.47 ExposureTime = 0.745675 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -40 420 66.8751 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:09:50 FilterSlitAndLoss = 20 0 [ZValue = 46] TiltAngle = 31.9915 StagePosition = -215.531 -315.997 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -9.93716 ImageShift = -0.0634688 0.172573 RotationAngle = 175.47 ExposureTime = 0.76698 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -40 456 66.6792 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:09:55 FilterSlitAndLoss = 20 0 [ZValue = 47] TiltAngle = 33.9937 StagePosition = -215.533 -315.99 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.0321 ImageShift = -0.0659118 0.175528 RotationAngle = 175.47 ExposureTime = 0.788285 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -40 446 66.5953 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:10:16 FilterSlitAndLoss = 20 0 [ZValue = 48] TiltAngle = 35.9925 StagePosition = -215.546 -315.985 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.0367 ImageShift = -0.0580685 0.159947 RotationAngle = 175.47 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -41 593 65.2163 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:10:20 FilterSlitAndLoss = 20 0 [ZValue = 49] TiltAngle = 37.9937 StagePosition = -215.551 -315.976 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.0596 ImageShift = -0.0499167 0.144789 RotationAngle = 175.47 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 455 64.1287 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:10:36 FilterSlitAndLoss = 20 0 [ZValue = 50] TiltAngle = 39.9785 StagePosition = -215.489 -315.992 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.1067 ImageShift = -0.0549558 0.152648 RotationAngle = 175.47 ExposureTime = 0.8522 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -45 503 65.187 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:11:11 FilterSlitAndLoss = 20 0 [ZValue = 51] TiltAngle = 41.9933 StagePosition = -215.53 -315.965 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.194 ImageShift = -0.0817973 0.249933 RotationAngle = 175.47 ExposureTime = 0.873505 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -41 872 64.9001 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:11:38 FilterSlitAndLoss = 20 0 [ZValue = 52] TiltAngle = 43.993 StagePosition = -215.571 -315.967 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.2907 ImageShift = -0.135466 0.387816 RotationAngle = 175.47 ExposureTime = 0.905463 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -38 518 64.9006 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:12:02 FilterSlitAndLoss = 20 0 [ZValue = 53] TiltAngle = 45.9933 StagePosition = -215.602 -315.968 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.3812 ImageShift = -0.177957 0.502539 RotationAngle = 175.47 ExposureTime = 0.93742 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -34 551 64.542 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:12:14 FilterSlitAndLoss = 20 0 [ZValue = 54] TiltAngle = 47.9915 StagePosition = -215.577 -315.975 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.4732 ImageShift = -0.104029 0.365312 RotationAngle = 175.47 ExposureTime = 0.969378 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 1736 61.4243 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:12:33 FilterSlitAndLoss = 20 0 [ZValue = 55] TiltAngle = 49.9928 StagePosition = -215.574 -315.971 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.4681 ImageShift = -0.183069 0.506639 RotationAngle = 175.47 ExposureTime = 1.01199 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -31 681 62.4922 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:12:55 FilterSlitAndLoss = 20 0 [ZValue = 56] TiltAngle = 51.9915 StagePosition = -215.575 -315.968 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.5387 ImageShift = -0.0382361 0.220771 RotationAngle = 175.47 ExposureTime = 1.0546 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -33 559 57.8119 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:13:12 FilterSlitAndLoss = 20 0 [ZValue = 57] TiltAngle = 53.9928 StagePosition = -215.578 -315.964 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.6051 ImageShift = -0.124059 0.375159 RotationAngle = 175.47 ExposureTime = 1.10786 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -36 598 58.7715 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:13:24 FilterSlitAndLoss = 20 0 [ZValue = 58] TiltAngle = 55.992 StagePosition = -215.57 -315.949 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.6714 ImageShift = -0.0592157 0.231359 RotationAngle = 175.47 ExposureTime = 1.16112 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -33 583 55.4944 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:13:40 FilterSlitAndLoss = 20 0 [ZValue = 59] TiltAngle = 57.9793 StagePosition = -215.478 -315.943 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.3207 ImageShift = -0.0227095 0.140512 RotationAngle = 175.47 ExposureTime = 1.22504 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -39 539 53.9407 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:14:25 FilterSlitAndLoss = 20 0 [ZValue = 60] TiltAngle = 59.992 StagePosition = -215.517 -315.923 StageZ = 140.373 Magnification = 26000 Intensity = 0.124328 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -10.6146 ImageShift = -0.104386 0.295072 RotationAngle = 175.47 ExposureTime = 1.29961 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 25 CountsPerElectron = 16 MinMaxMean = -37 605 56.2672 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 23-Jun-19 10:14:53 FilterSlitAndLoss = 20 0