PixelSpacing = 4.494 Voltage = 300 ImageFile = Hpylori_dFliO15.mrc ImageSize = 5760 4092 DataMode = 1 [T = SerialEM: Acquired by K3 on Caltech Krios 21-Sep-19 20:40:41 ] [T = Tilt axis angle = 84.3, binning = 1 spot = 5 camera = 0 bidir = 0.0] [ZValue = 0] TiltAngle = 49.9823 StagePosition = -272.999 -10.1442 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6569 ImageShift = 0.177924 -0.403059 RotationAngle = 174.34 ExposureTime = 1.24634 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -34 1289 207.58 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:40:41 FilterSlitAndLoss = 30 0 [ZValue = 1] TiltAngle = 47.982 StagePosition = -272.953 -10.1715 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6017 ImageShift = 0.210489 -0.454067 RotationAngle = 174.34 ExposureTime = 1.19308 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -27 730 211.689 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:40:17 FilterSlitAndLoss = 30 0 [ZValue = 2] TiltAngle = 45.9813 StagePosition = -272.937 -10.1589 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.7942 ImageShift = 0.151978 -0.339702 RotationAngle = 174.34 ExposureTime = 1.15047 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -40 1759 206.464 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:39:54 FilterSlitAndLoss = 30 0 [ZValue = 3] TiltAngle = 43.9805 StagePosition = -272.933 -10.1809 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8046 ImageShift = 0.13751 -0.302557 RotationAngle = 174.34 ExposureTime = 1.11851 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -45 1070 206.39 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:39:49 FilterSlitAndLoss = 30 0 [ZValue = 4] TiltAngle = 41.9813 StagePosition = -272.935 -10.1704 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8203 ImageShift = 0.115586 -0.257204 RotationAngle = 174.34 ExposureTime = 1.0759 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -36 1257 203.869 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:39:32 FilterSlitAndLoss = 30 0 [ZValue = 5] TiltAngle = 39.9805 StagePosition = -272.918 -10.1704 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8389 ImageShift = 0.103869 -0.231414 RotationAngle = 174.34 ExposureTime = 1.04395 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -42 758 203.606 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:39:11 FilterSlitAndLoss = 30 0 [ZValue = 6] TiltAngle = 37.9817 StagePosition = -272.912 -10.1736 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8444 ImageShift = 0.089461 -0.209445 RotationAngle = 174.34 ExposureTime = 1.01199 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -48 691 202.055 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:39:06 FilterSlitAndLoss = 30 0 [ZValue = 7] TiltAngle = 35.982 StagePosition = -272.909 -10.183 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8498 ImageShift = 0.0998426 -0.215451 RotationAngle = 174.34 ExposureTime = 0.990683 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -30 732 202.446 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:39:01 FilterSlitAndLoss = 30 0 [ZValue = 8] TiltAngle = 33.9837 StagePosition = -272.909 -10.1883 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8439 ImageShift = 0.113684 -0.240754 RotationAngle = 174.34 ExposureTime = 0.969378 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -41 1128 203.147 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:38:56 FilterSlitAndLoss = 30 0 [ZValue = 9] TiltAngle = 31.982 StagePosition = -272.9 -10.1914 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.9129 ImageShift = 0.109101 -0.244542 RotationAngle = 174.34 ExposureTime = 0.948072 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 755 202.848 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:38:27 FilterSlitAndLoss = 30 0 [ZValue = 10] TiltAngle = 29.9852 StagePosition = -272.893 -10.2134 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.9049 ImageShift = 0.0963987 -0.204295 RotationAngle = 174.34 ExposureTime = 0.926768 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -34 668 200.723 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:38:23 FilterSlitAndLoss = 30 0 [ZValue = 11] TiltAngle = 27.9835 StagePosition = -272.892 -10.2124 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8945 ImageShift = 0.0816776 -0.177124 RotationAngle = 174.34 ExposureTime = 0.905463 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -33 1181 199.329 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:38:18 FilterSlitAndLoss = 30 0 [ZValue = 12] TiltAngle = 25.9822 StagePosition = -272.885 -10.2186 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8845 ImageShift = 0.083747 -0.179145 RotationAngle = 174.34 ExposureTime = 0.89481 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -35 835 200.141 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:37:56 FilterSlitAndLoss = 30 0 [ZValue = 13] TiltAngle = 23.984 StagePosition = -272.884 -10.2354 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8785 ImageShift = 0.0683508 -0.152326 RotationAngle = 174.34 ExposureTime = 0.873505 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -30 1136 197.683 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:37:43 FilterSlitAndLoss = 30 0 [ZValue = 14] TiltAngle = 21.9832 StagePosition = -272.877 -10.2469 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8767 ImageShift = 0.0578054 -0.13064 RotationAngle = 174.34 ExposureTime = 0.862853 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -42 654 198.313 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:37:31 FilterSlitAndLoss = 30 0 [ZValue = 15] TiltAngle = 19.9824 StagePosition = -272.863 -10.2417 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8443 ImageShift = 0.0420821 -0.0897714 RotationAngle = 174.34 ExposureTime = 0.8522 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 635 197.286 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:37:08 FilterSlitAndLoss = 30 0 [ZValue = 16] TiltAngle = 17.9817 StagePosition = -272.866 -10.2365 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8427 ImageShift = 0.0290931 -0.0784884 RotationAngle = 174.34 ExposureTime = 0.841547 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -31 641 197.789 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:36:55 FilterSlitAndLoss = 30 0 [ZValue = 17] TiltAngle = 15.9824 StagePosition = -272.867 -10.2721 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.841 ImageShift = 0.0146615 -0.0378303 RotationAngle = 174.34 ExposureTime = 0.830895 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -36 846 196.301 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:36:51 FilterSlitAndLoss = 30 0 [ZValue = 18] TiltAngle = 13.9832 StagePosition = -272.859 -10.2595 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8388 ImageShift = -0.00707072 0.0152669 RotationAngle = 174.34 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -41 640 195.318 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:36:35 FilterSlitAndLoss = 30 0 [ZValue = 19] TiltAngle = 11.9829 StagePosition = -272.864 -10.2585 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8522 ImageShift = -0.0260003 0.0392088 RotationAngle = 174.34 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 701 196.68 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:36:14 FilterSlitAndLoss = 30 0 [ZValue = 20] TiltAngle = 9.98417 StagePosition = -272.859 -10.2658 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8452 ImageShift = -0.0140606 0.0305239 RotationAngle = 174.34 ExposureTime = 0.80959 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -29 700 195.114 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:36:09 FilterSlitAndLoss = 30 0 [ZValue = 21] TiltAngle = 7.98242 StagePosition = -272.855 -10.2448 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.836 ImageShift = -0.0124499 0.0341796 RotationAngle = 174.34 ExposureTime = 0.80959 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -29 677 195.865 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:36:04 FilterSlitAndLoss = 30 0 [ZValue = 22] TiltAngle = 5.98416 StagePosition = -272.866 -10.2511 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8527 ImageShift = -0.00897044 0.0293124 RotationAngle = 174.34 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -40 646 193.616 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:35:42 FilterSlitAndLoss = 30 0 [ZValue = 23] TiltAngle = 3.98441 StagePosition = -272.862 -10.271 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8303 ImageShift = -0.0248588 0.0521564 RotationAngle = 174.34 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 621 193.821 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:35:36 FilterSlitAndLoss = 30 0 [ZValue = 24] TiltAngle = 1.98465 StagePosition = -272.87 -10.2952 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8127 ImageShift = -0.0288168 0.0540112 RotationAngle = 174.34 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -36 641 193.847 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:35:13 FilterSlitAndLoss = 30 0 [ZValue = 25] TiltAngle = -0.00860156 StagePosition = -272.865 -10.3884 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.7879 ImageShift = -0.0356321 0.0532529 RotationAngle = 174.34 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -28 640 194.711 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:34:50 FilterSlitAndLoss = 30 0 [ZValue = 26] TiltAngle = -1.99386 StagePosition = -272.804 -10.642 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8911 ImageShift = -0.0274191 0.134401 RotationAngle = 174.34 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -29 623 195.173 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:42:01 FilterSlitAndLoss = 30 0 [ZValue = 27] TiltAngle = -3.99211 StagePosition = -272.802 -10.6997 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8734 ImageShift = -0.0459134 0.152788 RotationAngle = 174.34 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -43 643 194.671 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:42:23 FilterSlitAndLoss = 30 0 [ZValue = 28] TiltAngle = -5.99187 StagePosition = -272.805 -10.684 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8406 ImageShift = -0.0543823 0.169713 RotationAngle = 174.34 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -36 1036 194.653 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:42:46 FilterSlitAndLoss = 30 0 [ZValue = 29] TiltAngle = -7.99112 StagePosition = -272.8 -10.6798 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.8178 ImageShift = -0.0795425 0.212183 RotationAngle = 174.34 ExposureTime = 0.80959 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -28 625 196.634 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:42:51 FilterSlitAndLoss = 30 0 [ZValue = 30] TiltAngle = -9.99188 StagePosition = -272.807 -10.6777 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.7885 ImageShift = -0.0949049 0.255441 RotationAngle = 174.34 ExposureTime = 0.80959 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 657 195.324 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:43:13 FilterSlitAndLoss = 30 0 [ZValue = 31] TiltAngle = -11.9921 StagePosition = -272.814 -10.6504 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.7595 ImageShift = -0.125096 0.308744 RotationAngle = 174.34 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -27 627 196.588 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:43:18 FilterSlitAndLoss = 30 0 [ZValue = 32] TiltAngle = -13.9919 StagePosition = -272.816 -10.6745 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.7316 ImageShift = -0.118785 0.30675 RotationAngle = 174.34 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -33 750 195.235 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:43:23 FilterSlitAndLoss = 30 0 [ZValue = 33] TiltAngle = -15.9921 StagePosition = -272.818 -10.6473 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.7243 ImageShift = -0.13687 0.346244 RotationAngle = 174.34 ExposureTime = 0.830895 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -31 634 196.108 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:43:44 FilterSlitAndLoss = 30 0 [ZValue = 34] TiltAngle = -17.9919 StagePosition = -272.824 -10.642 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6998 ImageShift = -0.162243 0.38241 RotationAngle = 174.34 ExposureTime = 0.841547 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -26 651 197.247 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:44:00 FilterSlitAndLoss = 30 0 [ZValue = 35] TiltAngle = -19.9922 StagePosition = -272.827 -10.6504 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6766 ImageShift = -0.164598 0.409544 RotationAngle = 174.34 ExposureTime = 0.8522 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -31 625 197.398 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:44:05 FilterSlitAndLoss = 30 0 [ZValue = 36] TiltAngle = -21.9924 StagePosition = -272.833 -10.6358 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6703 ImageShift = -0.149823 0.389899 RotationAngle = 174.34 ExposureTime = 0.862853 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -55 665 198.094 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:44:27 FilterSlitAndLoss = 30 0 [ZValue = 37] TiltAngle = -23.9922 StagePosition = -272.838 -10.619 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6489 ImageShift = -0.154604 0.39113 RotationAngle = 174.34 ExposureTime = 0.873505 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -38 650 198.052 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:44:32 FilterSlitAndLoss = 30 0 [ZValue = 38] TiltAngle = -25.9814 StagePosition = -272.842 -10.5037 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6242 ImageShift = -0.182846 0.438876 RotationAngle = 174.34 ExposureTime = 0.884158 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 835 197.456 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:45:08 FilterSlitAndLoss = 30 0 [ZValue = 39] TiltAngle = -27.9917 StagePosition = -272.844 -10.5739 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.626 ImageShift = -0.161477 0.419583 RotationAngle = 174.34 ExposureTime = 0.905463 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 740 199.19 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:45:36 FilterSlitAndLoss = 30 0 [ZValue = 40] TiltAngle = -29.9904 StagePosition = -272.845 -10.641 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.6055 ImageShift = -0.165808 0.426892 RotationAngle = 174.34 ExposureTime = 0.926768 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -34 675 200.034 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:46:05 FilterSlitAndLoss = 30 0 [ZValue = 41] TiltAngle = -31.9912 StagePosition = -272.844 -10.5949 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.5999 ImageShift = -0.212152 0.518461 RotationAngle = 174.34 ExposureTime = 0.93742 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -27 764 198.645 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:46:21 FilterSlitAndLoss = 30 0 [ZValue = 42] TiltAngle = -33.9894 StagePosition = -272.86 -10.5938 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.5906 ImageShift = -0.220689 0.532265 RotationAngle = 174.34 ExposureTime = 0.969378 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -42 1559 201.477 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:46:26 FilterSlitAndLoss = 30 0 [ZValue = 43] TiltAngle = -35.9907 StagePosition = -272.862 -10.5917 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.449 ImageShift = -0.235212 0.571101 RotationAngle = 174.34 ExposureTime = 0.990683 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -39 1342 200.138 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:46:49 FilterSlitAndLoss = 30 0 [ZValue = 44] TiltAngle = -37.9894 StagePosition = -272.878 -10.5519 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.4065 ImageShift = -0.255799 0.606577 RotationAngle = 174.34 ExposureTime = 1.01199 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -36 694 199.982 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:46:54 FilterSlitAndLoss = 30 0 [ZValue = 45] TiltAngle = -39.9887 StagePosition = -272.88 -10.5896 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.3605 ImageShift = -0.252926 0.615191 RotationAngle = 174.34 ExposureTime = 1.04395 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 679 200.845 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:46:58 FilterSlitAndLoss = 30 0 [ZValue = 46] TiltAngle = -41.9875 StagePosition = -272.883 -10.5247 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.3144 ImageShift = -0.25857 0.611264 RotationAngle = 174.34 ExposureTime = 1.0759 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -36 1123 201.427 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:47:15 FilterSlitAndLoss = 30 0 [ZValue = 47] TiltAngle = -43.9907 StagePosition = -272.887 -10.5561 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.2357 ImageShift = -0.262733 0.616233 RotationAngle = 174.34 ExposureTime = 1.10786 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -35 774 200.751 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:47:37 FilterSlitAndLoss = 30 0 [ZValue = 48] TiltAngle = -45.9875 StagePosition = -272.898 -10.4901 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.1834 ImageShift = -0.285695 0.628745 RotationAngle = 174.34 ExposureTime = 1.15047 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -34 702 201.555 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:47:42 FilterSlitAndLoss = 30 0 [ZValue = 49] TiltAngle = -47.9762 StagePosition = -272.899 -10.4136 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.136 ImageShift = -0.282065 0.596337 RotationAngle = 174.34 ExposureTime = 1.19308 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -37 1740 202.22 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:48:18 FilterSlitAndLoss = 30 0 [ZValue = 50] TiltAngle = -49.989 StagePosition = -272.901 -10.4241 StageZ = -10.3176 Magnification = 19500 Intensity = 0.136181 ExposureDose = 0 PixelSpacing = 4.494 SpotSize = 5 Defocus = -14.2169 ImageShift = -0.211829 0.473419 RotationAngle = 174.34 ExposureTime = 1.24634 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 MagIndex = 24 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -57 759 204.098 TargetDefocus = -8 PriorRecordDose = 0 DateTime = 21-Sep-19 20:48:46 FilterSlitAndLoss = 30 0