PixelSpacing = 3.36 Voltage = 300 ImageFile = on17-1_lam3_ts2.mrc ImageSize = 5760 4092 DataMode = 1 [T = SerialEM: Acquired by K3 on Caltech Krios 13-Feb-20 17:04:13 ] [T = Tilt axis angle = 85.5, binning = 1 spot = 6 camera = 0 bidir = 0.0] [ZValue = 0] TiltAngle = -59.9903 StagePosition = -89.0465 -419.986 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.2697 ImageShift = -0.963105 1.97867 RotationAngle = 175.47 ExposureTime = 1.59787 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -5 1991 377.858 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:04:08 FilterSlitAndLoss = 20 0 [ZValue = 1] TiltAngle = -56.9891 StagePosition = -88.9774 -420 StageZ = 25.2242 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.0428 ImageShift = -0.946862 1.94739 RotationAngle = 175.47 ExposureTime = 1.47004 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -9 1203 365.25 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:03:46 FilterSlitAndLoss = 20 0 [ZValue = 2] TiltAngle = -53.9905 StagePosition = -88.8966 -420.016 StageZ = 25.2242 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -14.98 ImageShift = -0.92779 1.89304 RotationAngle = 175.47 ExposureTime = 1.36352 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -18 1081 353.211 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:03:33 FilterSlitAndLoss = 20 0 [ZValue = 3] TiltAngle = -50.9919 StagePosition = -88.8188 -420.031 StageZ = 25.2242 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -14.868 ImageShift = -0.892318 1.81153 RotationAngle = 175.47 ExposureTime = 1.26765 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -15 1383 339.296 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:03:12 FilterSlitAndLoss = 20 0 [ZValue = 4] TiltAngle = -47.9917 StagePosition = -88.7536 -420.041 StageZ = 25.2242 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -14.693 ImageShift = -0.849885 1.73993 RotationAngle = 175.47 ExposureTime = 1.19308 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -12 875 329.709 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:02:51 FilterSlitAndLoss = 20 0 [ZValue = 5] TiltAngle = -44.9911 StagePosition = -88.7565 -420.047 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -14.7423 ImageShift = -0.820651 1.69233 RotationAngle = 175.47 ExposureTime = 1.12917 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -8 1758 319.72 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:02:38 FilterSlitAndLoss = 20 0 [ZValue = 6] TiltAngle = -41.9925 StagePosition = -88.6991 -420.072 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -14.8536 ImageShift = -0.776405 1.61476 RotationAngle = 175.47 ExposureTime = 1.0759 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -21 1986 309.689 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:02:17 FilterSlitAndLoss = 20 0 [ZValue = 7] TiltAngle = -38.9928 StagePosition = -88.6903 -420.085 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -14.8945 ImageShift = -0.740594 1.57687 RotationAngle = 175.47 ExposureTime = 1.03329 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -29 1899 302.253 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:02:07 FilterSlitAndLoss = 20 0 [ZValue = 8] TiltAngle = -35.9942 StagePosition = -88.6484 -420.101 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -14.9281 ImageShift = -0.708549 1.50117 RotationAngle = 175.47 ExposureTime = 0.990683 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -19 1436 296.927 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:01:53 FilterSlitAndLoss = 20 0 [ZValue = 9] TiltAngle = -32.9946 StagePosition = -88.6076 -420.106 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.0341 ImageShift = -0.658294 1.39688 RotationAngle = 175.47 ExposureTime = 0.948072 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -24 1355 283.598 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:01:33 FilterSlitAndLoss = 20 0 [ZValue = 10] TiltAngle = -29.9949 StagePosition = -88.5258 -420.117 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.0565 ImageShift = -0.618007 1.2925 RotationAngle = 175.47 ExposureTime = 0.926768 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -28 1085 281.422 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:01:19 FilterSlitAndLoss = 20 0 [ZValue = 11] TiltAngle = -26.9953 StagePosition = -88.4392 -420.115 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.0759 ImageShift = -0.563971 1.1508 RotationAngle = 175.47 ExposureTime = 0.89481 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -29 1457 272.537 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:01:06 FilterSlitAndLoss = 20 0 [ZValue = 12] TiltAngle = -23.9937 StagePosition = -88.4236 -420.113 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.1007 ImageShift = -0.490884 1.0051 RotationAngle = 175.47 ExposureTime = 0.873505 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -29 821 265.528 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:00:49 FilterSlitAndLoss = 20 0 [ZValue = 13] TiltAngle = -20.995 StagePosition = -88.4324 -420.105 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.1287 ImageShift = -0.403708 0.830774 RotationAngle = 175.47 ExposureTime = 0.8522 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -35 903 259.63 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:00:44 FilterSlitAndLoss = 20 0 [ZValue = 14] TiltAngle = -17.9939 StagePosition = -88.4402 -420.096 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.1576 ImageShift = -0.314718 0.669538 RotationAngle = 175.47 ExposureTime = 0.841547 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -30 1270 255.249 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:00:27 FilterSlitAndLoss = 20 0 [ZValue = 15] TiltAngle = -14.9953 StagePosition = -88.4596 -420.07 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.1775 ImageShift = -0.225815 0.503495 RotationAngle = 175.47 ExposureTime = 0.830895 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -37 1639 256.745 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:00:17 FilterSlitAndLoss = 20 0 [ZValue = 16] TiltAngle = -11.9961 StagePosition = -88.4664 -420.065 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.1889 ImageShift = -0.162266 0.374039 RotationAngle = 175.47 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -26 1123 254.108 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:00:12 FilterSlitAndLoss = 20 0 [ZValue = 17] TiltAngle = -8.995 StagePosition = -88.4664 -420.045 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.2346 ImageShift = -0.0979829 0.245225 RotationAngle = 175.47 ExposureTime = 0.80959 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -28 1263 251.694 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 16:59:53 FilterSlitAndLoss = 20 0 [ZValue = 18] TiltAngle = -5.99637 StagePosition = -88.4713 -420.041 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.2287 ImageShift = -0.0373138 0.119555 RotationAngle = 175.47 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -31 1434 248.433 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 16:59:28 FilterSlitAndLoss = 20 0 [ZValue = 19] TiltAngle = -2.99674 StagePosition = -88.4694 -420.007 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.5067 ImageShift = 0.0221266 -0.0124099 RotationAngle = 175.47 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -33 1384 247.373 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 16:59:04 FilterSlitAndLoss = 20 0 [ZValue = 20] TiltAngle = 0.00239709 StagePosition = -88.4986 -419.861 StageZ = 25.2235 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -13.882 ImageShift = 0.00849026 -0.0409115 RotationAngle = 175.47 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -30 1250 248.071 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 16:58:46 FilterSlitAndLoss = 20 0 [ZValue = 21] TiltAngle = 2.98453 StagePosition = -88.5424 -419.792 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.2795 ImageShift = 0.327918 -0.491104 RotationAngle = 175.47 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -35 749 251.066 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:05:24 FilterSlitAndLoss = 20 0 [ZValue = 22] TiltAngle = 5.98216 StagePosition = -88.5433 -419.776 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.4554 ImageShift = 0.373669 -0.630446 RotationAngle = 175.47 ExposureTime = 0.798937 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -39 957 248.523 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:05:44 FilterSlitAndLoss = 20 0 [ZValue = 23] TiltAngle = 8.9828 StagePosition = -88.5355 -419.756 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.4459 ImageShift = 0.451708 -0.782756 RotationAngle = 175.47 ExposureTime = 0.80959 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -31 1266 249.84 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:06:04 FilterSlitAndLoss = 20 0 [ZValue = 24] TiltAngle = 11.9819 StagePosition = -88.5394 -419.74 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.4075 ImageShift = 0.507058 -0.891477 RotationAngle = 175.47 ExposureTime = 0.820243 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -35 1131 249.71 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:06:23 FilterSlitAndLoss = 20 0 [ZValue = 25] TiltAngle = 14.9821 StagePosition = -88.5404 -419.72 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.3884 ImageShift = 0.570881 -1.02449 RotationAngle = 175.47 ExposureTime = 0.830895 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -36 1411 248.982 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:06:28 FilterSlitAndLoss = 20 0 [ZValue = 26] TiltAngle = 17.9827 StagePosition = -88.5355 -419.683 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.3536 ImageShift = 0.639344 -1.15834 RotationAngle = 175.47 ExposureTime = 0.841547 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -32 813 245.536 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:06:45 FilterSlitAndLoss = 20 0 [ZValue = 27] TiltAngle = 20.9823 StagePosition = -88.5375 -419.653 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.3217 ImageShift = 0.79227 -1.46142 RotationAngle = 175.47 ExposureTime = 0.8522 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -24 641 253.014 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:06:55 FilterSlitAndLoss = 20 0 [ZValue = 28] TiltAngle = 23.9825 StagePosition = -88.5375 -419.614 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.4074 ImageShift = 0.770131 -1.42173 RotationAngle = 175.47 ExposureTime = 0.873505 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -39 1041 247.873 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:07:15 FilterSlitAndLoss = 20 0 [ZValue = 29] TiltAngle = 26.9841 StagePosition = -88.5443 -419.594 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.3894 ImageShift = 0.791801 -1.45988 RotationAngle = 175.47 ExposureTime = 0.89481 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -30 779 242.087 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:07:26 FilterSlitAndLoss = 20 0 [ZValue = 30] TiltAngle = 29.9847 StagePosition = -88.5443 -419.556 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.3447 ImageShift = 0.959467 -1.79721 RotationAngle = 175.47 ExposureTime = 0.926768 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -23 1471 251.404 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:07:45 FilterSlitAndLoss = 20 0 [ZValue = 31] TiltAngle = 32.9854 StagePosition = -88.5385 -419.513 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.3619 ImageShift = 0.937283 -1.76509 RotationAngle = 175.47 ExposureTime = 0.958725 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -31 1812 242.094 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:07:57 FilterSlitAndLoss = 20 0 [ZValue = 32] TiltAngle = 35.9845 StagePosition = -88.5404 -419.479 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.3597 ImageShift = 0.994967 -1.87427 RotationAngle = 175.47 ExposureTime = 0.990683 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -28 2152 237.272 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:08:02 FilterSlitAndLoss = 20 0 [ZValue = 33] TiltAngle = 38.9826 StagePosition = -88.5453 -419.445 StageZ = 25.2249 Magnification = 26000 Intensity = 0.035121 ExposureDose = 0 PixelSpacing = 3.36 SpotSize = 6 Defocus = -15.4586 ImageShift = 1.04735 -1.98577 RotationAngle = 175.47 ExposureTime = 1.03329 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 MinMaxMean = -30 1181 228.321 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 13-Feb-20 17:08:19 FilterSlitAndLoss = 20 0