PixelSpacing = 3.332 Voltage = 300 ImageFile = on29-2_lam5_ts1.mrc ImageSize = 5760 4092 DataMode = 1 [T = SerialEM: Acquired by K3 on Caltech Krios 10-Nov-20 15:09:21 ] [T = Tilt axis angle = 84.7, binning = 1 spot = 5 camera = 0 bidir = 0.0] [ZValue = 0] MinMaxMean = -37 346 55.7378 TiltAngle = 38.9851 StagePosition = -629.61 -36.8462 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 6.79508 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.38002 ImageShift = -0.665991 1.34053 RotationAngle = 174.73 ExposureTime = 0.617845 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:09:13 FilterSlitAndLoss = 20 0 [ZValue = 1] MinMaxMean = -38 712 73.5144 TiltAngle = 35.9865 StagePosition = -629.618 -36.8714 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 9.49657 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.46229 ImageShift = -0.662498 1.31604 RotationAngle = 174.73 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:08:59 FilterSlitAndLoss = 20 0 [ZValue = 2] MinMaxMean = -42 413 78.4798 TiltAngle = 32.9869 StagePosition = -629.611 -36.8871 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 10.6094 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.55175 ImageShift = -0.652142 1.28896 RotationAngle = 174.73 ExposureTime = 0.575235 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:08:30 FilterSlitAndLoss = 20 0 [ZValue = 3] MinMaxMean = -43 402 82.4197 TiltAngle = 29.9837 StagePosition = -629.612 -36.9091 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 11.6692 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.63055 ImageShift = -0.663795 1.30874 RotationAngle = 174.73 ExposureTime = 0.55393 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:08:08 FilterSlitAndLoss = 20 0 [ZValue = 4] MinMaxMean = -35 474 93.8603 TiltAngle = 26.9836 StagePosition = -629.623 -36.9437 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 13.7688 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.70913 ImageShift = -0.443864 0.862984 RotationAngle = 174.73 ExposureTime = 0.543278 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:07:45 FilterSlitAndLoss = 20 0 [ZValue = 5] MinMaxMean = -44 1091 101.841 TiltAngle = 23.982 StagePosition = -629.614 -36.9563 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 15.7899 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.31827 ImageShift = -0.237194 0.451291 RotationAngle = 174.73 ExposureTime = 0.521973 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:07:21 FilterSlitAndLoss = 20 0 [ZValue = 6] MinMaxMean = -40 473 101.993 TiltAngle = 20.9823 StagePosition = -629.596 -36.9206 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 16.1923 PixelSpacing = 3.332 SpotSize = 5 Defocus = 0.879181 ImageShift = -0.16718 0.212164 RotationAngle = 174.73 ExposureTime = 0.51132 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:07:04 FilterSlitAndLoss = 20 0 [ZValue = 7] MinMaxMean = -37 445 100.255 TiltAngle = 17.9837 StagePosition = -629.577 -33.6729 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 16.2651 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.50568 ImageShift = -1.29502 2.47495 RotationAngle = 174.73 ExposureTime = 0.500668 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:06:09 FilterSlitAndLoss = 20 0 [ZValue = 8] MinMaxMean = -43 332 100.651 TiltAngle = 14.9826 StagePosition = -629.575 -33.6792 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 16.3384 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.45456 ImageShift = -1.07119 2.03722 RotationAngle = 174.73 ExposureTime = 0.500668 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:06:04 FilterSlitAndLoss = 20 0 [ZValue = 9] MinMaxMean = -38 527 98.4968 TiltAngle = 11.9829 StagePosition = -629.572 -33.7043 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 16.3375 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.33113 ImageShift = -0.846841 1.59812 RotationAngle = 174.73 ExposureTime = 0.490015 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:05:47 FilterSlitAndLoss = 20 0 [ZValue = 10] MinMaxMean = -42 464 97.9352 TiltAngle = 8.9868 StagePosition = -629.573 -33.7305 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 16.2316 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.3163 ImageShift = -0.627224 1.16285 RotationAngle = 174.73 ExposureTime = 0.490015 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:05:23 FilterSlitAndLoss = 20 0 [ZValue = 11] MinMaxMean = -34 481 95.6615 TiltAngle = 5.98766 StagePosition = -629.57 -33.7714 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 16.2055 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.2967 ImageShift = -0.402974 0.713632 RotationAngle = 174.73 ExposureTime = 0.479363 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:04:57 FilterSlitAndLoss = 20 0 [ZValue = 12] MinMaxMean = -44 589 95.7225 TiltAngle = 2.99003 StagePosition = -629.56 -33.7924 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 16.2172 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.91993 ImageShift = -0.215683 0.341921 RotationAngle = 174.73 ExposureTime = 0.479363 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:04:29 FilterSlitAndLoss = 20 0 [ZValue = 13] MinMaxMean = -40 402 94.5792 TiltAngle = -0.00460205 StagePosition = -629.557 -33.829 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 15.9976 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.44911 ImageShift = -0.0497978 -0.0297003 RotationAngle = 174.73 ExposureTime = 0.479363 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:04:12 FilterSlitAndLoss = 20 0 [ZValue = 14] MinMaxMean = -41 422 92.785 TiltAngle = -2.99524 StagePosition = -629.535 -33.7096 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 15.6544 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.86556 ImageShift = 0.0106869 0.0238841 RotationAngle = 174.73 ExposureTime = 0.479363 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:10:53 FilterSlitAndLoss = 20 0 [ZValue = 15] MinMaxMean = -45 466 91.3942 TiltAngle = -5.99637 StagePosition = -629.535 -33.7305 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 15.3896 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.18532 ImageShift = 0.186863 -0.338036 RotationAngle = 174.73 ExposureTime = 0.479363 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:11:10 FilterSlitAndLoss = 20 0 [ZValue = 16] MinMaxMean = -41 534 91.2485 TiltAngle = -8.9965 StagePosition = -629.538 -33.741 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 14.9847 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.40059 ImageShift = 0.399101 -0.738902 RotationAngle = 174.73 ExposureTime = 0.490015 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:11:38 FilterSlitAndLoss = 20 0 [ZValue = 17] MinMaxMean = -38 557 88.9452 TiltAngle = -11.9961 StagePosition = -629.538 -33.7546 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 14.5605 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.57404 ImageShift = 0.602591 -1.12019 RotationAngle = 174.73 ExposureTime = 0.490015 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:12:06 FilterSlitAndLoss = 20 0 [ZValue = 18] MinMaxMean = -37 874 88.0491 TiltAngle = -14.9963 StagePosition = -629.543 -33.7588 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 14.0528 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.72278 ImageShift = 0.837803 -1.57561 RotationAngle = 174.73 ExposureTime = 0.500668 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:12:26 FilterSlitAndLoss = 20 0 [ZValue = 19] MinMaxMean = -39 600 84.8704 TiltAngle = -17.9964 StagePosition = -629.547 -33.7672 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 13.4888 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.88801 ImageShift = 0.997195 -1.86596 RotationAngle = 174.73 ExposureTime = 0.500668 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:12:38 FilterSlitAndLoss = 20 0 [ZValue = 20] MinMaxMean = -41 610 82.909 TiltAngle = -20.995 StagePosition = -629.55 -33.7724 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 12.8391 PixelSpacing = 3.332 SpotSize = 5 Defocus = -3.04904 ImageShift = 1.24357 -2.32924 RotationAngle = 174.73 ExposureTime = 0.51132 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:12:53 FilterSlitAndLoss = 20 0 [ZValue = 21] MinMaxMean = -48 591 80.6093 TiltAngle = -23.9947 StagePosition = -629.55 -33.7787 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 12.1658 PixelSpacing = 3.332 SpotSize = 5 Defocus = -3.35475 ImageShift = 1.42849 -2.6795 RotationAngle = 174.73 ExposureTime = 0.521973 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:13:15 FilterSlitAndLoss = 20 0 [ZValue = 22] MinMaxMean = -47 500 79.7029 TiltAngle = -26.9948 StagePosition = -629.501 -30.072 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 11.4976 PixelSpacing = 3.332 SpotSize = 5 Defocus = 1.0911 ImageShift = 0.066459 -0.146189 RotationAngle = 174.73 ExposureTime = 0.543278 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:14:08 FilterSlitAndLoss = 20 0 [ZValue = 23] MinMaxMean = -43 572 75.5637 TiltAngle = -29.9959 StagePosition = -629.485 -29.9871 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 10.6197 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.31615 ImageShift = 0.2197 -0.405285 RotationAngle = 174.73 ExposureTime = 0.55393 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:14:30 FilterSlitAndLoss = 20 0 [ZValue = 24] MinMaxMean = -41 2478 72.6546 TiltAngle = -32.9956 StagePosition = -629.491 -29.9797 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 9.76805 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.6998 ImageShift = 0.435849 -0.805916 RotationAngle = 174.73 ExposureTime = 0.575235 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:14:59 FilterSlitAndLoss = 20 0 [ZValue = 25] MinMaxMean = -40 2867 68.7109 TiltAngle = -35.9952 StagePosition = -629.49 -29.9724 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 8.8442 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.60234 ImageShift = 0.662068 -1.22926 RotationAngle = 174.73 ExposureTime = 0.59654 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:15:27 FilterSlitAndLoss = 20 0 [ZValue = 26] MinMaxMean = -35 3269 63.8422 TiltAngle = -38.9943 StagePosition = -629.489 -29.964 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 7.87411 PixelSpacing = 3.332 SpotSize = 5 Defocus = -1.77298 ImageShift = 0.895533 -1.67627 RotationAngle = 174.73 ExposureTime = 0.617845 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:15:55 FilterSlitAndLoss = 20 0 [ZValue = 27] MinMaxMean = -37 3247 58.7659 TiltAngle = -41.995 StagePosition = -629.488 -29.9598 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 6.83454 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.22679 ImageShift = 1.14426 -2.15048 RotationAngle = 174.73 ExposureTime = 0.649803 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:16:23 FilterSlitAndLoss = 20 0 [ZValue = 28] MinMaxMean = -45 693 51.9427 TiltAngle = -44.9936 StagePosition = -629.487 -29.9577 StageZ = 45.9252 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 5.70494 PixelSpacing = 3.332 SpotSize = 5 Defocus = -2.98574 ImageShift = 1.44362 -2.72819 RotationAngle = 174.73 ExposureTime = 0.68176 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:16:49 FilterSlitAndLoss = 20 0 [ZValue = 29] MinMaxMean = -46 3273 44.4519 TiltAngle = -47.9937 StagePosition = -629.482 -29.9514 StageZ = 45.9259 Magnification = 26000 Intensity = 0.0371713 ExposureDose = 0 DoseRate = 4.62049 PixelSpacing = 3.332 SpotSize = 5 Defocus = -3.41872 ImageShift = 1.69774 -3.22092 RotationAngle = 174.73 ExposureTime = 0.713718 Binning = 1 CameraIndex = 0 DividedBy2 = 1 OperatingMode = 1 UsingCDS = 1 MagIndex = 25 LowDoseConSet = 4 CountsPerElectron = 16 TargetDefocus = -10 PriorRecordDose = 0 DateTime = 10-Nov-20 15:17:05 FilterSlitAndLoss = 20 0